Investigation of B-Z transitions with DNA oligonucleotides containing 8-methylguanine
نویسندگان
چکیده
منابع مشابه
Synthesis, structure and thermodynamic properties of 8-methylguanine-containing oligonucleotides: Z-DNA under physiological salt conditions.
Various oligonucleotides containing 8-methylguanine (m8G) have been synthesized and their structures and thermodynamic properties investigated. Introduction Of M8G into DNA sequences markedly stabilizes the Z conformation under low salt conditions. The hexamer d(CGC[M8G]CG)2 exhibits a CD spectrum characteristic of the Z conformation under physiological salt conditions. The NOE-restrained refin...
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O-Methylguanine (OmeG) is one of the most toxic, mutagenic, and carcinogenic lesions caused by the interaction of DNA with several catabolism products as well as with environmental methylating agents. Carcinogenic impact of OmeG can be conditioned not only by its mutagenic properties but also by alteration in enzymatic methylation of the C5 carbon atom of cytosine residue in CpG sequences. In t...
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Herein, we report on the use of a tricyclic cytosine FRET pair, incorporated into DNA with different base pair separations, to study Z-DNA and B-Z DNA junctions. With its position inside the DNA structure, the FRET pair responds to a B- to Z-DNA transition with a distinct change in FRET efficiency for each donor/acceptor configuration allowing reliable structural probing. Moreover, we show how ...
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Under negative torsion, DNA adopts left-handed helical forms, such as Z-DNA and L-DNA. Using the random copolymer model developed for a wormlike chain, we represent a single DNA molecule with structural heterogeneity as a helical chain consisting of monomers which can be characterized by different helical senses and pitches. By Monte Carlo simulation, where we take into account bending and twis...
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The preparation of oligonucleotides containing 8-bromo-2'-deoxyguanosine is described. Substitution of G by 8-bromoguanine on an alternating CG decamer stabilizes the Z-form in such a way that the B-form was not observed. Melting temperatures showed that duplexes in which 8-bromo-2'-deoxyguanosine paired with natural bases were much less stable.
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ژورنال
عنوان ژورنال: Artificial DNA: PNA & XNA
سال: 2014
ISSN: 1949-095X,1949-0968
DOI: 10.4161/adna.28226